
Jung-Ja Kim, PhD
Emeritus Professor
Locations
- Biochemistry
TBRC C2960
Contact Information
Education
Biography
Dr. Kim received her PhD in Physical Chemistry from Cornell University and her postdoctoral study at Massachusetts Institute of Technology involved the structure determination of transfer RNA. After a brief interruption of her career, she joined the faculty of the Medical College of Wisconsin in 1978.
Research Interests
Our research interest is to study the structure-function relationship of biologically interesting molecules by using X-ray diffraction methods, one of the most powerful techniques to date to study macromolecular structure. Currently our studies are focused on the following projects:
(Image: Ribbon structure of the MCAD tetramer complexed with C8-CoA)
Acyl-CoA Dehydrogenases and Related Enzymes
Acyl-CoA Dehydrogenases are a family of enzymes that are involved in both the first oxidative step in the metabolism of fatty acids and in the catabolism of some amino acids. Electron transfer from the primary dehydrogenase to the main mitochondrial respiratory chain is catalyzed, in sequence, by electron transfer flavoprotein (ETF) and the membrane-associated ETF-ubiquinone oxidoreductase (ETF-QO). The crystal structures of several acyl-CoA dehydrogenases including medium chain acyl-CoA dehydrogenase, short chain acyl-CoA dehydrogenase, and isovaleryl-CoA dehydrogenase have been determined in our laboratory. These structures reveal their catalytic mechanism as well as the structural basis for the substrate specificity. Currently we are extending these studies to site-specific mutants, inhibitor/substrate complexes, and to other members of the dehydrogenase family.
We have recently obtained high resolution structures of human ETF and a bacterial ETF. These, together with those of various acyl-CoA dehydrogenases, have enabled us to study the molecular basis of electron transfer between the dehydrogenases and ETF and of flavoprotein-flavoprotein interactions, in general. We have recently crystallized the membrane associated protein, ETF-QO and its complete structure determination is in progress.
(Image: Ribbon diagram of NADPH-Cytochrome P450 oxidoreductase)
NADPH-Cytochrome P450 oxidoreductase
NADPH-cytochrome P450 reductase dancing with cytochrome P450 Movie
NADPH-Cytochrome P450 Reductase exists in every tissue in which cytochrome P450-mediated reactions occur of both endogenous substrates, including steroids, fatty acids, and prostaglandins, and exogenous compounds such as therapeutic drugs, environmental toxicants, and carcinogens. We have recently solved the three dimensional structure of the rat liver reductase. The structure shows how the two flavins (FMN and FAD) are communicating with each other and provides insights into not only the interaction of the reductase with its physiological electron partner, cytochrome P450, but also the mechanism of electron transfer and its regulation in other FMN- and FAD-containing enzymes, including nitric oxide synthase isozymes. We are extending our studies to interactions between cytochromes P450 and the reductase, as well as to other related enzymes.
Structure/Function studies of Mannose 6-Phosphate Receptors (MPRs)Kim Structure 3
MPRs are responsible for the targeting of lysosomal acid hydrolases to lysosomes. In collaboration with Dr. Nancy Dahms, we have been studying the structure/function relationships of these receptors. We have solved the structures of the cation dependent MPR (CD-MPR) with and without mannose 6-phosphate ligand and complexes with high mannose oligosaccharides. Currently, we are extending our studies to the larger of the two receptors, the insulin-like growth factor II/cation-independent MPR (IGF-II/CI-MPR).
Publications
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(Narayanan B, Xia C, McAndrew R, Shen AL, Kim JP.) Sci Rep. 2024 Jun 05;14(1):12976 PMID: 38839792 PMCID: PMC11153573 06/06/2024
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(Narayanan B, Xia C, McAndrew R, Shen AL, Kim JP.) Res Sq. 2024 Feb 29 PMID: 38464032 PMCID: PMC10925408 03/11/2024
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(Xia C, Lou B, Fu Z, Mohsen AW, Shen AL, Vockley J, Kim JP.) iScience. 2021 Oct 22;24(10):103153 PMID: 34646991 PMCID: PMC8497999 10/15/2021
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(Hubbard PA, Xia C, Shen AL, Kim JP.) Arch Biochem Biophys. 2021 Apr 15;701:108792 PMID: 33556357 PMCID: PMC8020834 02/09/2021
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(Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM.) Commun Biol. 2020 Sep 09;3(1):498 PMID: 32908216 PMCID: PMC7481795 SCOPUS ID: 2-s2.0-85090384984 09/11/2020
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(Xia C, Shen AL, Duangkaew P, Kotewong R, Rongnoparut P, Feix J, Kim JP.) Biochemistry. 2019 May 14;58(19):2408-2418 PMID: 31009206 PMCID: PMC6873807 SCOPUS ID: 2-s2.0-85065650755 04/23/2019
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Crystal structure of human mitochondrial trifunctional protein, a fatty acid β-oxidation metabolon.
(Xia C, Fu Z, Battaile KP, Kim JP.) Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):6069-6074 PMID: 30850536 PMCID: PMC6442613 03/10/2019
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(Xia C, Rwere F, Im S, Shen AL, Waskell L, Kim JP.) Biochemistry. 2018 Feb 13;57(6):945-962 PMID: 29308883 PMCID: PMC5967631 01/09/2018
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(Galiakhmetov AR, Kovrigina EA, Xia C, Kim JP, Kovrigin EL.) J Biomol NMR. 2018 Jan;70(1):21-31 PMID: 29168021 PMCID: PMC5820150 11/24/2017
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(Kovrigina EA, Pattengale B, Xia C, Galiakhmetov AR, Huang J, Kim JP, Kovrigin EL.) Biochemistry. 2016 Nov 01;55(43):5973-5976 PMID: 27741572 PMCID: PMC6505697 11/02/2016
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(McCammon KM, Panda SP, Xia C, Kim JJ, Moutinho D, Kranendonk M, Auchus RJ, Lafer EM, Ghosh D, Martasek P, Kar R, Masters BS, Roman LJ.) J Biol Chem. 2016 Sep 23;291(39):20487-502 PMID: 27496950 PMCID: PMC5034044 08/09/2016
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Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function.
(Floyd BJ, Wilkerson EM, Veling MT, Minogue CE, Xia C, Beebe ET, Wrobel RL, Cho H, Kremer LS, Alston CL, Gromek KA, Dolan BK, Ulbrich A, Stefely JA, Bohl SL, Werner KM, Jochem A, Westphall MS, Rensvold JW, Taylor RW, Prokisch H, Kim JP, Coon JJ, Pagliarini DJ.) Mol Cell. 2016 Aug 18;63(4):621-632 PMID: 27499296 PMCID: PMC4992456 08/09/2016