Linda Berg Luecke, PhD

Mass Spectrometry, Cell Surface Glycoproteins in Advanced Heart Failure

Research Experience

Medical College of Wisconsin, 2018-2022
Advisor: Rebekah Gundry, PhD
Project: Cell surface and extracellular glycoproteins in advanced heart failure (7/1/2019-6/302020: CTSI, TL1 7/1/2020-6/30/2020: AHA predoctoral fellowship)

MD Anderson Cancer Center
Advisor: Raghu Kalluri, MD, PhD, 2013-2014
Project: Biology of exosomes and their association with cancer development and metastasis. Tumor-derived exosomes’ utility as circulating biomarker in early detection of cancer.

Versiti Blood Center of Wisconsin, Blood Research Institute
Advisor: Subramaniam Malarkannan, PhD, 2014-2016
Project: Chimeric Antigen Receptor (CAR)-based NK cell-mediated cellular immunotherapy.

Publications

Berg Luecke LB, Waas M, Littrell J, Wojtkiewicz M, Castro C, Burkovetskaya M, Schuette EN, Buchberger AR, Churko JM, Chalise U, Waknitz M, Konfrst S, Teuben R, Morrissette-McAlmon J, Mahr C, Anderson DR, Boheler KR, Gundry RL. Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts. Nat Cardiovasc Res. 2023 Jan;2(1):76-95. doi: 10.1038/s44161-022-00200-y. Epub 2023 Jan 16. PMID: 36950336; PMCID: PMC10030153.

Young JB, Buchberger AR, Bogaard, JD, Berg Luecke L, Runquist M, Skumatx CMB, Kassem IS. Tissue plasminogen activator effects on fibron volume and the ocular proteome in a juvenile rabbit model of lensectomy. Trans Vis Sci Technol. 2021; 10(14). PMID 34874448.

Wojtkiewicz M, Berg Luecke L, Castro C, Burkovetskaya M, Mesidor R, Gundry RL, Bottom-up proteomic analysis of human adult cardiac tissue and isolated cardiomyocytes, Journal of Molecular and Cellular Cardiology, 162:20-31, 2021. PMID: 34437879.

Whyte ML, Smith KA, Buchberger A, Berg Luecke L, Tjan LH, Mori Y, Gundry RL, Hudson AW. The Roseoloviruses Downregulate the Protein Tyrosine Phosphatase PTPRC (CD45). J Virol. 2021 Jun 24;95(14):e0162820. doi: 10.1128/JVI.01628-20. Epub 2021 Jun 24. PMID: 33952641; PMCID: PMC8223955.

Wojtkiewicz M, Berg Luecke L, Kelly MI, Gundry RL. Facile Preparation of Peptides for Mass Spectrometry Analysis in Bottom-Up Proteomics Workflows. Curr Protoc. 2021 Mar;1(3):e85. doi: 10.1002/cpz1.85. PMID: 33750040; PMCID: PMC8011989.

Berg Luecke L, Gundry RL. Assessment of Streptavidin Bead Binding Capacity to Improve Quality of Streptavidin-based Enrichment Studies. J Proteome Res. 2021 Feb 5;20(2):1153-1164. doi: 10.1021/acs.jproteome.0c00772. Epub 2020 Dec 3. PMID: 33270449.

Berg Luecke L, Waas M, Gundry RL Reliable Protocols for Flow Cytometry Analysis of Intracellular Proteins in Pluripotent Stem Cell Derivatives: A Fit-For-Purpose Approach. Current Protocols in Stem Cell Biology, 2019 Sep;50(1):e94, 2019. PMID: 31479597

Melo S.A., Luecke, L.B., Kahlert, C., Fernandez, A., Gammon, S.T, Kaye J., LeBleu, V.S, Mittendorf E.A., Weitz J., Rahbari N., Reissfelder, C., Fraga, M., Piwnica-Worms, D. and Kalluri, K. (2015). Identification of GPC1 as a Specific Biomarker for Circulating Cancer Exosomes with Utility for Detection of Early Stage Pancreas Cancer. Nature 523, 177-182.

Sunchu, B., Berg, L., Ward, H. E., Lopper, M. E. (2012). Identification of a small molecule PriA helicase inhibitor. Biochemistry 51, 10137-46. 

Meeting Abstracts

Berg Luecke L, Waas M, Littrell J, Gundry RL. CellSurfer platform reveals the first experimentally derived surfaceome map of primary human cardiac cells, US HUPO 2022, Charleston, SC, February 2022.

Berg Luecke L, Gundry RL. Cell surface phenotyping of the human heart reveals cardiomyocyte-specific targets and surfaceome dynamics of explanted cardiac fibroblast, HUPO ReCONNECT 2021, Virtual, November 2021.

Berg Luecke L, Waas M, Gundry RL. CellSurfer platform for semi-automated cell surface N-glycoproteins profiling of human primary cells reveals chamber-specific cardiomyocyte surface maps, Midland Society of Physiological Sciences, Virtual, October 2021. 

General Interests

Spending time with family, traveling, yoga