Brian Smith, PhD

Associate Director, Program in Chemical Biology; Associate Professor, Biochemistry

Locations

Biochemistry
BSB 356

Contact Information

Education

PhD, University of Wisconsin - Madison, 2008
BS, University of Illinois at Urbana-Champaign, 2003

Please see the Smith lab website for more information

Biography

Dr. Smith received his Bachelor of Science in Chemistry from the University of Illinois at Urbana-Champaign in 2003. He then received his PhD in Chemistry from the University of Wisconsin-Madison in 2008, where his research focused on the chemical mechanisms of sirtuin protein lysine deacetylases. During his graduate career he spent a brief period at Genentech in the Department of Medicinal Chemistry where he synthesized drugs that target the Inhibitor of Apoptosis family of proteins. From 2009-2014, Dr. Smith performed postdoctoral studies on nitric oxide synthases and cysteine S-nitrosation at the University of California, Berkeley and the Scripps Research Institute in La Jolla. During his postdoctoral studies Dr. Smith was awarded an NIH Ruth L. Kirschstein National Research Service Award. Dr. Smith joined the faculty at the Medical College of Wisconsin in 2014.

                
                    Research Experience
                
                    Amino Acids, Peptides, and Proteins
Binding Sites
Biochemistry
Catalysis
Deuterium Exchange Measurement
Enzyme Activators
Enzyme Inhibitors
Enzymes and Coenzymes
Histone Code
Histone Deacetylases
Mass Spectrometry
Molecular Probes

                    Methodologies and Techniques
                
                    Biochemistry
Biological Assay
chemical synthesis
Chromatography, Affinity
Electrophoresis
enzymology
Mass Spectrometry
Models, Chemical
Models, Molecular
Mutagenesis
Nuclear Magnetic Resonance, Biomolecular
Peptide Library

                    Leadership Positions
                
                    Associate Director, Program in Chemical Biology
Associate Director, Structural Genomics Unit, Mellowes Center for Genomic Sciences and Precision Medicine

                    MCW Program / Core Facilities
                
                    Biochemistry
Redox Biology

Inhibition of Sirtuin Deacylase Activity by Peroxynitrite.

(Bohl K, Wynia-Smith SL, Jones Lipinski RA, Smith BC.) Biochemistry. 2024 Oct 01;63(19):2463-2476 PMID: 39256054 PMCID: PMC11524680 SCOPUS ID: 2-s2.0-85205075999 09/11/2024
Lipid Trolling to Optimize A3 Adenosine Receptor-Positive Allosteric Modulators (PAMs).

(Pradhan B, Pavan M, Fisher CL, Salmaso V, Wan TC, Keyes RF, Rollison N, Suresh RR, Kumar TS, Gao ZG, Smith BC, Auchampach JA, Jacobson KA.) J Med Chem. 2024 Jul 25;67(14):12221-12247 PMID: 38959401 SCOPUS ID: 2-s2.0-85197620924 07/03/2024
Oxidized LDL regulates efferocytosis through the CD36-PKM2-mtROS pathway.

(Zhang J, Chang J, Chen V, Beg MA, Huang W, Vick L, Wang Y, Zhang H, Yttre E, Gupta A, Castleberry M, Zhang Z, Dai W, Song S, Zhu J, Yang M, Brown AK, Xu Z, Ma YQ, Smith BC, Zielonka J, Traylor JG, Dhaou CB, Orr AW, Cui W, Zheng Z, Chen Y.) bioRxiv. 2024 Jul 15 PMID: 39071358 PMCID: PMC11275753 07/29/2024
Zinc-chelating BET bromodomain inhibitors equally target islet endocrine cell types.

(Jones Lipinski RA, Stancill JS, Nuñez R, Wynia-Smith SL, Sprague DJ, Nord JA, Bird A, Corbett JA, Smith BC.) Am J Physiol Regul Integr Comp Physiol. 2024 Jun 01;326(6):R515-R527 PMID: 38618911 PMCID: PMC11381023 SCOPUS ID: 2-s2.0-85194976711 04/15/2024
Cancer-associated polybromo-1 bromodomain 4 missense variants variably impact bromodomain ligand binding and cell growth suppression.

(Bursch KL, Goetz CJ, Jiao G, Nuñez R, Olp MD, Dhiman A, Khurana M, Zimmermann MT, Urrutia RA, Dykhuizen EC, Smith BC.) J Biol Chem. 2024 Apr;300(4):107146 PMID: 38460939 PMCID: PMC11002309 SCOPUS ID: 2-s2.0-85189184192 03/10/2024
Current Trends in Sirtuin Activator and Inhibitor Development.

(Bursch KL, Goetz CJ, Smith BC.) Molecules. 2024 Mar 06;29(5) PMID: 38474697 PMCID: PMC10934002 03/13/2024
Extrahelical Binding Site for a 1H-Imidazo[4,5-c]quinolin-4-amine A3 Adenosine Receptor Positive Allosteric Modulator on Helix 8 and Distal Portions of Transmembrane Domains 1 and 7.

(Fisher CL, Pavan M, Salmaso V, Keyes RF, Wan TC, Pradhan B, Gao ZG, Smith BC, Jacobson KA, Auchampach JA.) Mol Pharmacol. 2024 Feb 15;105(3):213-223 PMID: 38182432 PMCID: PMC10877738 SCOPUS ID: 2-s2.0-85185222449 01/06/2024
Identification of Protein Targets of S-Nitroso-Coenzyme A-Mediated S-Nitrosation Using Chemoproteomics.

(Falco JA, Wynia-Smith SL, McCoy J, Smith BC, Weerapana E.) ACS Chem Biol. 2024 Jan 19;19(1):193-207 PMID: 38159293 PMCID: PMC11154738 01/02/2024
Deep computational phenotyping of genomic variants impacting the SET domain of KMT2C reveal molecular mechanisms for their dysfunction.

(Jorge SD, Chi YI, Mazaba JL, Haque N, Wagenknecht J, Smith BC, Volkman BF, Mathison AJ, Lomberk G, Zimmermann MT, Urrutia R.) Front Genet. 2023;14:1291307 PMID: 38090150 PMCID: PMC10715303 SCOPUS ID: 2-s2.0-85179362461 12/13/2023
A multi-layered computational structural genomics approach enhances domain-specific interpretation of Kleefstra syndrome variants in EHMT1.

(Chi YI, Jorge SD, Jensen DR, Smith BC, Volkman BF, Mathison AJ, Lomberk G, Zimmermann MT, Urrutia R.) Comput Struct Biotechnol J. 2023;21:5249-5258 PMID: 37954151 PMCID: PMC10632586 11/13/2023
Fragment-based screening by protein-detected NMR spectroscopy.

(Kerber PJ, Nuñez R, Jensen DR, Zhou AL, Peterson FC, Hill RB, Volkman BF, Smith BC.) Methods Enzymol. 2023;690:285-310 PMID: 37858532 PMCID: PMC10657026 SCOPUS ID: 2-s2.0-85166280504 10/20/2023
Analysis of continuous enzyme kinetic data using ICEKAT.

(Bursch KL, Olp MD, Smith BC.) Methods Enzymol. 2023;690:109-129 PMID: 37858527 PMCID: PMC10691744 10/20/2023

Brian Smith, PhD

Associate Director, Program in Chemical Biology; Associate Professor, Biochemistry

Locations

Contact Information

Education

Biography

Research Experience

Methodologies and Techniques

Leadership Positions

MCW Program / Core Facilities

Publications