Julian Grosskopf

Structural Biology & Biophysics

Research Experience

Medical College of Wisconsin, 2021-present
Advisor: Michael Lerch, PhD
Project: 1) Exploring the molecular mechanisms of lipid head group interactions with the beta-2 adrenergic receptor, 2) Molecular modeling of protein conformational ensembles from DEER spectroscopy data, 3) Using pressure jump EPR spectroscopy to evaluate kinetics of protein conformational rearrangement

University of Wisconsin – La Crosse 2016-2019
Advisors: Drs. Todd Weaver & Daniel Grilley
Projects: 1) Performing site-directed alterations to the hemolysin A non polar core to probe for critical residues of structural and functional importance concerning red blood cell lysis, 2) Calculating and extrapolating chemical and thermal stability of SS loop mutants within fumarase C as part of a larger project in exploring the SS loop and its importance in catalysis of fumarate to S-malate. 

Publications

Grosskopf JD, Sidabras JW, Altenbach C, Anderson JR, Mett RR, Strangeway RA, Hyde JS, Hubbell WL, Lerch MT. A pressure-jump EPR system to monitor millisecond conformational exchange rates of spin-labeled proteins. bioRxiv [Preprint]. 2024 May 11:2024.05.07.593074. doi: 10.1101/2024.05.07.593074. PMID: 38766191; PMCID: PMC11100676.

Stuttgen GM, Grosskopf JD, Berger CR, May JF, Bhattacharyya B, Weaver TM. Closed fumarase C active-site structures reveal SS Loop residue contribution in catalysis. FEBS Lett. 2020 Jan;594(2):337-357. doi: 10.1002/1873-3468.13603. Epub 2019 Sep 23. PMID: 31514245. 

Meeting Abstracts

Grosskopf JD, Sidabras JW, Altenbach C, Anderson JR, Mett RR, Strangeway RA, Hyde JS, Hubbell WL, Lerch MT. Characterization of Protein Conformational Exchange Kinetics Using Pressure-jump EPR. 2023. https://doi.org/10.56902/RMCMR.2023.63.1. [Poster]

Weaver T, Stuttgen G, Grosskopf J, Berger C, May J, Bhattacharyya B. Alterations within fumarase C reveal newly observed SS Loop conformations. 2019. FASEB J. 33(S1), 468.7-468.7. https://dx.doi.org/10.1096/fasebj.2019.33.1_supplement.468.7. [Poster]

Grosskopf J, Weaver T, Grilley D. Site-selective alterations within the hemolysin A non-polar core. FASEB J. 32(S1), 792.17-792.17. 2018. https://doi.org/10.1096/fasebj.2018.32.1_supplement.792.17. [Poster]

General Interests

Archery, computer science, functional fitness, and enjoying the outdoors with friends and family