Medical College of Wisconsin Center for Immunology Shared Resources

The Seahorse XF Pro Analyzer measures mitochondrial respiration, glycolosis, and ATP production in live cells in a 96-well format. 

Manager: Donna McAllister

Location: TBRC 3rd Floor C3773

The Incucyte S3 derives deeper and more physiologically relevant informaiton about your cells - plus real-time kinetic data. It automatically acquires and analyzes images around the clock, providing an information-rich analysis that is easy to achieve.

Manager: Donna McAllister

Location: TBRC 3rd Floor C3773

Find a Mentor

Graduate trainees, postdoctoral fellows, and clinical fellows looking for assistance in finding an immunology mentor can reach out to the Center for Immunology. View current faculty members  

Model Systems

• Mouse models
• B-cell
• Human Studies
• T-cell
• Mast cells
• Fibroblasts
• Epithelial
• Plasma Cells
• Innate Lymphoid Cells

Techniques

• Chromatography
• ELISA
• Flow Cytometry
• Imaging
• Mass-spectrometry
• HPLC
• Multiplex
• Immune Assays
• Autoradiography
• NMR
• X-ray Crystallography
• RNA Sequencing
• Incucyte or Cell Based Assays

Research Focus

• Autoimmunity
• Solid Tumors
• Inflammatory
• Infectious Disease - Bacterial & Viral
• Hematologic Cancers
• Transplantation
• Microbiome
• Molecular Profiling
• Host Defense

 

The Center for Immunology Consulting and Mentoring Service provides an online survey to process and expedite your inquiry. All members are invited to use the following link to submit their request directly to the Center. Once submitted, the Center will best align your needs to one of our consultants or mentors.  Our consultants are internal and external with broad experience in all areas of focus in immunology. 

Consulting and Mentoring Survey

Bryon Johnson, PhD | Director, Cell Therapy Lab
Bjohnson@mcw.edu

Tyce Kearl, MD, PhD | Assistant Director, Cell Therapy Lab
tkearl@mcw.edu

Berkeley Lights Lightning Optofluidics Platform
This is a novel optofluidic platform that allows one to visualize the phenotype & function of individual cells on a microchip that contains 1500 chambers. 

Isoplexis
This chip-based platform analyzes single cells for the production of 32 human cytokines or 28 mouse cytokines and effector molecules including effector, stimulatory, chemoattractive, regulatory and inflammatory mediators.

Luminex Flexmap 3D
The FLEXMAP 3D® system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to enable precise, flexible, rapid multiplexing of up to 500 unique assays within a single sample.

MACSQuant 10 Flow Cytometer
This flow cytometer is a 10-parameter, 8-color flow cytometry analyzer that uses a CAR T Cell Express Mode Package design or one can create their own flow cytometry panels.

xCELLigence RTCA 6-Plate System
This instrument allows for measurement of immune cell cytotoxicity or cell death in a 96-well format.

Learn more about the Cell Therapy Lab's shared resources.

Calvin Williams, MD, PhD | Director, CRI Lab
cbwillia@mcw.edu

Galina Petrova, PhD | FLOW Core Manager
gpetrova@mcw.edu

FACSAria II
The FACSAria II operator-assisted high speed cell sorting in both standard and BSL-2+ modes. It has a 13 color 15-parameter custom-made high-speed digital bench-top cell sorter equipped with four adjustable excitation lasers (100 mW Blue, 50 mW 561nm Green, 100 mW Red and 100 mW violet).

LSRII
The LSRII is a multi-laser flow cytometer that utilizes fixed-alignment lasers that transmit light reflected by mirrors through a flow cell to user-defined octagon and trigon arrays. These fluorescence signals are collected and translated to electronic signals then converted to digital data.

FACSCalibur
A two-laser (488nm, 615 nm), four-color, six-parameter, bench-top analyzer used primarily for evaluating surface and intracellular antigen expression, apoptosis detection, bead-based cytokine detection and cell-cycle analysis.

LSRFortessa X20
The analyzer can be configured with up to 5 lasers to detect up to 20 parameters simultaneously to support ever increasing demands in multicolor flow cytometry. A wide range of up to 34 laser choices is available as excitation sources, including blue, red, violet, yellow-green, and UV.

Wolf Cell Sorter
The sorter uses a totally different approach compared to conventional jet-in-air cell sorters. This is critical for sensitive cells like Stem Cells where high pressures can damage cells and affect their biological behavior. With an on-chip fast valve, NanoCellect’s sorting mechanism gently guides cells into collection channels.

Chromium Single Cell Gene Expression- 10X Genomics
Chromium Single Cell Immune Profiling analyzes full length paired B-cell or T-cell receptors, surface protein expression, antigen specificity, and gene expression, all from a single cell.

William Cashdollar, PhD | Director, Versiti Research Core Labs & Facilities
lwcashdollar@versiti.org

Benedetta Bonacci | Flow Core Laboratory Operator
benedetta.bonacci@bcw.edu

Brad Best | Viral Vector Senior Research Technologist
brad.best@bcw.edu

Erin Yttre | Hybridoma & Microscopic Imaging Core Laboratory Operator
erin.yttre@bcw.edu

Marie Schulte, PhD | Microscopic Imaging Core Laboratory Operator
marie.schulte@bcw.edu

Flow Cytometry Core
Flow Cytometry Core contains Becton Dickinson cytometer systems; BD LSRII Cytometer, BD FACS Celesta, BD FACSAria Cell Sorter, BD FACS Melody Cell Sorter and Accuri C6 - Learn more about the Flow Cytometry Core

Viral Vector Core
Viral Vector Core provides services in the areas of vector design, gene silencing and protein expression, including construction, amplification, purification and titration - Learn more about the Viral Vector Core

Hybridoma Core
Hybridoma Core provides services and products related to the production of monoclonal antibodies.Basic procedures include mouse immunization, with immunogen provided by requestor. Subsequent boost and bleeds are done to check antibody titer in the animal. Spleen cells are fused with a non immunoglobulin-secreting mouse myeloma cell line - Learn more about the Hybridoma Core

Single Cell Core

Microscopic Imaging
Microscopic Imaging Core provides researchers access to a wide variety of microscope systems. The Core Lab staff trains users and provides guidance with image acquisition and analysis - Learn more about the Microscopic Imaging Core

Learn more about the Versiti Research Core Lab

IRRADIATOR CORE

L. William Cashdollar, PhD | Director
bill.cashdollar@bcw.edu

IN VIVO IMAGING

Amit Joshi, PhD | Director
ajoshi@mcw.edu

Spectrum CT
IVIS 100

Center for Infectious Disease Research 

Neuroscience Research Center

Biostatistics

Transgenic Expression

CXCR3a; CXCR3b; CXCR3a/b expressing Molt 4; Jurkat; SupT1; & Hh cell lines
Dwinell Lab 

tdTomato/luciferase THP-1 & tdTomato/luciferase U-937
Dwinell Lab 

Eukaryotic Organisms

C. albicans- several lab strains, KO strains, and transgenic strains
Huppler Lab 

C. glabrata- reference strain
Huppler Lab 

C. auris- multiple strains from CDC
Huppler Lab

Knockout (Global)

CXCR3 KO
Dwinell Lab 

IFNAR KO
Tarakanova Lab 

IRF-1 KO
Tarakanova Lab 

IRF-3 KO
Tarakanova Lab 

IRF-7 KO
Tarakanova Lab 

IL-1R1 KO
Tarakanova Lab 

IL-10 KO
Lochhead Lab 

IL12p40 KO
Kroner-Milsch Lab (VA transfer)

IL12RB2 KO
Kroner-Milsch Lab (VA transfer) 

IL17ra KO
Huppler Lab 

IL23 KO
Huppler Lab 

IL23p19 KO ( MTA from Genentech)
Kroner-Milsch Lab (VA transfer) 

Hemopexin KO
Kroner-Milsch Lab (VA transfer) 

CCL3 KO
Kroner-Milsch Lab (VA transfer) 

CCL28 KO
Huppler Lab 

Knockout (Conditional)

KRas G12D; p53 mutant; p48-Cre
Dwinell and Lomberk Labs 

STAT1fl/fl (crossed to LyzM-Cre, CD19-Cre, or dLck-Cre)
Tarakanova Lab 

IRF-1fl/fl (crossed to CD19-Cre or dLck-Cre)
Tarakanova Lab 

IL-1R1 fl/fl (crossed to CD19-Cre or dLck-Cre)
Tarakanova Lab

Shp1 fl/fl (crossed to CD19-Cre)
Tarakanova Lab 

Transgenic

MHC-II reporter B6
Lochhead Lab

Biostatistics Consulting Services include assistance with design and analysis of clinical trials, observational studies, surveys, assistance with public databases, sample size and power calculations and interpretation.

learn about the Bioconsulting Consulting Service

The Center for Biomedical Mass Spectrometry Research (MS Center) can assist investigators in completing simple projects using predefined fee-for-service workflows. This unique collaborative model enables them to offer a broader range of customized analysis types beyond what you might find in a typical “core” facility.

Learn about Biomedical Mass Spectrometry Research

The Mellowes Center invites investigators of all disciplines and experience levels to a complimentary consultation with their subject matter experts.

Learn about the Mellowes Center

Brian Volkman, PhD | Director
bvolkman@mcw.edu

Brian Smith | Associate Director
Brismith@mcw.edu

The Program in Chemical Biology (PCB) provides resources in structure-based drug design, protein production, and organic synthesis to the MCW community for chemical biology and medicinal chemistry projects. The PCB encourages investigators interested in the development and use of small molecules for basic and translational research to take advantage of its capabilities which include; small-molecule library screening using NMR and other biophysical techniques, recombinant protein expression and purification, organic synthesis, and computational docking and homology modeling of proteins and small-molecule: protein interactions

Learn about the Program in Chemical Biology